Agia Galini is 61 km southeast of Rethymno and 68 km southwest of Heraklion, about midway along the south coast of Crete.

Agia Galini, with its strange but apt name (meaning "Saint Serenity", or "Holy Peace"), is a small, labyrinthine village built in the shape of an amphitheatre and surrounded by three mountains: Asideroto, Kedros and Psiloritis. It opens out into a pretty, picturesque harbour which is particularly busy in summer.

The small old houses of the village seem squashed by the larger apartments springing up in the area and the hotels in Agia Galini, built in an effort to accommodate rising numbers of visitors.

Agia Galini is a famous resort with a good beach, one of the first tourist resorts in Crete.